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Accurate stochastic fluorescence
spectroscopy The accurate stochastic fluorescence spectroscopy (ASFS) is a technology using laser induced fluorescence in a confocal opti- cal system. Freely diffusing labeled molecules in the sample emit characteristic fluorescence patterns ("fingerprints") that are used to detect and identify the specific molecules at the single molecule level. Any change on the labeled molecule, like binding reactions, aggregations or integration into membranes changes the mole- cule size and the brightness of the label and thus the finger- print. These effects are exploited to detect and quantify reac- tions and allow to study interactions in in-vivo-like conditions without surface immobilization and in difficult matrices. ASFS is a patent pending technology based on a new theory to fluorescence fluctuation and algorithms that replace conventional approaches such as fluorescence correlation spectroscopy or photon counting histogram methods. A detailed description of the ASFS method is available in the download section. |  |